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VEGETATIVE PROPAGATION OF CANNABIS BY STEM CUTTINGS

Deron Caplan

May 4, 2018

Vegetative propagation of cannabis by stem cuttings: effects of leaf number, cutting position, rooting hormone, and leaf tip removal


Read full study here


Best Way to Take Cannabis Cuttings

If you're cloning cannabis using stem cuttings, you’ve probably heard a lot of conflicting advice—how many leaves to leave on, whether to cut the leaf tips, and what kind of rooting hormone to use. This study took a scientific look at all of those factors to figure out what actually works.


What was tested:

Researchers took cannabis cuttings and tried different combinations of:

  • Number of leaves (2 vs. 3)

  • Whether leaf tips were trimmed

  • Position on the mother plant (apical/top vs. basal/bottom)

  • Type of rooting hormone (a synthetic IBA gel vs. a natural willow extract)

After 12 days in a controlled environment, they measured how many cuttings rooted and how good the roots were.


Key takeaways:

  • Use synthetic rooting hormone (IBA): Cuttings treated with IBA rooted twice as often and had better quality roots than those treated with willow extract.

  • Don’t trim the leaf tips: Cutting the tips of the leaves reduced rooting success without improving anything. If you need to reduce leaf area, just use fewer full leaves.

  • More leaves = better root quality: Three-leaf cuttings developed 15% better root systems than two-leaf ones. But both rooted at about the same rate.

  • Position on the plant doesn’t matter much: It didn’t matter whether the cutting came from the top or bottom of the mother plant—unless you trimmed the leaf tips. In that case, bottom cuttings performed worse, likely because they had smaller leaves to begin with.


Bottom line: 

For better rooting when propagating cannabis:

  • Use three full, uncut leaves

  • Don’t trim the leaves

  • Use a synthetic IBA rooting hormone

  • Don’t worry too much about where on the plant you take the cutting

If you need to reduce leaf area to fit more cuttings or manage humidity, it’s better to remove whole leaves than to snip off the tips.


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